The measurement of cytokine levels is an essential tool in the study of inflammation. An enzyme-linked immunosorbent test (ELISA), is a low-cost, simple analytical tool that can provide both the specificity as well as the sensitivity needed for studying cytokines in vivo and in vitro.
This communication describes a method to create an indirect sandwich ELISA that can detect and quantify cytokines or other biomarkers with precision and accuracy. The use of sequential ELISA tests to analyze multiple cytokines in samples with small volumes is also described. You can buy cytokine ELISA kits via www.bosterbio.com/multiplex-elisa-kit-for-mouse-cytokine-inflammation-14-plex-mek1003-boster.html.
Cytokines are a cornerstone of any study that deals with inflammation, whether it is an in vitro cell culture system or an in vivo animal model. The cytokine profile as a whole and the relative abundance of one cytokine, and the endogenous inhibitors, define an inflammatory process that is in motion.
The cytokine profile can be used to identify the source of an injury, insult, or infection and to help stage the disease process. These studies focus on the ability to identify, quantify, and distinguish a single cytokine among a variety of biomolecules in a sample. The indirect sandwich enzyme-linked immunesorbent assay (ELISA) is one such method.
Cytokine assays assess the preparation and treatment for blood and tissue samples that are related to cytokines. Because cytokines play a pivotal role in the progression and regression of many diseases and are biomarkers for many others, cytokine assays are crucial in disease diagnosis and monitoring.